PRODUCTION, ISOLATION, IMMOBILIZATION AND APPLICATION OF GLUCOAMYLASE FROM RHIZOPUS ORYZAE
Abstract: Experiments were
conducted to find optimum medium and process conditions based on shake-flask
and laboratory fermentor scales to anticipate industrial scale for the
production of glucoamylase. Membrane filtration and ammonium sulfate
precipitation were tested for the enzyme concentration and isolation
respectively. The glucoamylase enzyme properties were determined and preference
to various starch substrates were checked. Immobilization within commercial
agar and alginate were done and their shelf-life were observed. The application
of free glucoamylase in saccharification of the various starches were also
investigated after being liquefied with commercial alpha-amylase.
Penulis: A.T. Karossi, L.Z.
Udin
Kode Jurnal: jpkimiadd090255
