PENGARUH SUMBER KARBON TERHADAP PRODUKSI ENZIM INULINFRUKTOTRANSFERASE DARI Nonomuraea sp
Abstract: The aims of this
research is to produce inulinfructotransferase enzyme with potentially benefit
of bacteria Actinomycetes which was isolated from Indonesia soils to produce
Difructoanhydride-Ill (DFA III), a compound has physiology activities to
enhance calsium absorption of rats. cows and human colons so that the materials
can be applied as antiosteoporosis. The processing of inulinfructotransferase
enzyme from Nonomuraea sp by
usingshakeflask level and study the influence of carbohydrate source were conducted.
This enzyme has optimum temperature at
65rtC,higher than optimum temperature which has found before and the activities
was stable at 70dc for 20 minutes. So
that the enzyme has potentially
applicable for industry scale. The results indicated that optimation condition
for processing inulinfructotransferase enzyme of Nonomuraea sp : temperature
sore. incubation time 48 hours.carbon
source of inulin 20 g/L, nitrogen source 5g/L, noyeast extract added, the enzyme
activities was 7,3 Unit/ml; However,when
medium contains yeast extract 0,2 gIL,the
inulinfructotransferase enzyme activity was 14,6 Unit/mL.
Penulis: Yetti Mulyati I, Sri
Pudjiraharti, Een Sri Endah, Tanaka tanaka, Teruo Sone, Asano K
Kode Jurnal: jpkimiadd110185
