PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus
Abstract: The purpose of this
research was to determine the effect of polysaccharide krestin (PSK) on the
testicular protein profiles and testosterone levels of Mus musculus with
variety of dosages. This research used a completely randomized design. It were
devide into four treatment group i.e. control group, PSK treatment at a dose
of 15, 30, 60 mg/kgBW. Each group had
six replications. Testicular proteins were isolated by flushing technique and
analized by SDS-PAGE. Testosterone levels were analized using ELISA technique
at wavelength 450 nm. Protein bands analysis showed that there were no
diversification between four treatments. Molecular weight of protein bands were
87, 63, 57, 35, and 30 kDa. The results of research showed that the
testosterone levels at dosage 60 mg/kgBW had significanly different with
control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of 60 mg/kgBW had lowest level at dosage, i.e.
25946.8 ρg/mL. It can be concluded that giving variety of dosages of
polysaccharide krestin did not affect to testicular protein profiles but giving
effect to testosterone levels of Mus musculus.
Keywords: Coriolus versicolor;
Mus musculus; polysaccharide krestin; testicular protein; testosterone
Penulis: Sri Puji Astuti
Wahyuningsih, Virid Gibson, Alfiah Hayati
Kode Jurnal: jpbiologidd160553