Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers
Abstrak: Both morphological
and protein studies reveal that differences are observed between resistant
Synedrella nodiflora against fomesafen and its susceptible wildtype. These are,
however, more or less influenced by environmental factors, so that molecular
analysis employing DNA markers is necessarily required. The methods involved total
genomic DNA extraction using modified CTAB protocol following Doyle & Doyle
(1990), RAPD marker amplification and visualization of RAPD markers prior to
data analysis. Ten RAPD markers were used, but only seven of them showed
polymorphism. Calculation of genetic
distance and variation was carried out employing PopGen software.
Based on the RAPD markers used in this study, it can be concluded that
genetic distance between susceptible and resistant S. nodiflora is higher than
that within susceptible samples supporting our previous morphological and
protein data, although genetic variation among susceptible individuals seems to
be significantly high.
Penulis: Murni Dwiati, Agus
Hery Susanto
Kode Jurnal: jpbiologidd150858
