MIX CULTURE INOCULANT PRODUCTION OF PHOSPHATE SOLUBILYZING AND INDOLE ACETIC ACID (IAA) PRODUCER RHIZOBACTERIA WITH AMBARAWA PEAT SOIL RAWAPENING AS CARRIER
ABSTRACT: Plant production
development is the main goals that do for increase the farming quality to
fulfill the man needed in food. One of the ways is intensive farming,
by using organic or inorganic fertilizer.
Phosphate is the essential for plants.
IAA is the necessary plant regulator for the root. Both phosphate and IAA need in plant
growing and production. Biological
fertilizer is fertilizer
with microbial as the main material. Bacillus
sp.DUCC-BR-K1.7, Bacillus sp. DUCC-BR-KI3, Bacillus sp.DUCC-BR-KI.2b and Pseudomonas fluorescens, Azotobacter
chroococcum, Azotobqcter vinelandii, Azospirillum sp. and Azotobacter
brazilensis are bacteria that can solubilization the phosphate
and IAA synthesize. Those bacteria can be used as inoculants or biological fertilizer
that put on carrier. One way to support
the aim is giving the altemative
carrier with suitable composition. The
carrier should be support bacterial life
during the storage. The aim
ofthis research is find
ths right consortia so can be
used to optimized viabilities of
culture Bacillus sp.
DUCC-BR-KIJ, Bacillus sp. DUCC-BR-K1.3,
Bacillus sp. DUCC-BR-KI.2b, P. flourescens, A. chroococcum, A. vinelandii,
Azospirillum sp., and A. brazilensis in mix culture
on peat soil as carrier. This
research done in Microbiology Laboratory of Biology Department Diponegoro
University. Subculture and activated culture in Nutrient Broth medium, make the growth curve to the
biomass production, make the inoculums, prepare the peat soil, biomass
production and mixed biomass with the
carrier, enumeration bacterial culture viability test in carrier during the storage by TPC method. The
results show that
all consortia culture
bacteria viabiliry Bacillus sp.
DUCC-BR-KI.3 with Azospirillum (A) and
Bacillus sp. DUCC-BR-K1.7, with P. flourescens (B) still viable and increasing number of
population during seven weeks storage with
l0 t-n CFU/g at To and up to l0
r3-ro CFU/g at the end storage. The consortium
C between Bacillus sp.
DUCC-BR-KI.2 b and A-
brssilensis increasing the
number of population and
still viable during eight weeks
storage (1013 - 1014
CFU/g).
Penulis: Budi Raharjo, Agung
Suprihadi
Kode Jurnal: jpmatematikadd100075
