Kinetika dan Karakterisasi Amiloglukosidase Isolat Aaspergillus niger Dari Limbah Ampas Sagu
Abstract: Study of characterization and kinetic
amyloglucosidase from Aspergillusniger isolate using sago pith waste has been
done. The aims of this research wereto know the optimumsubstrate concentration,
metalions effect, and value of KM and vmaks of amyoglucosidase. Theresult of
researches were obtained the optimum activity of amyoglucosidaseat 2% (w/v) ofstarch
concentration. The activator of amyoglucosidase were Zn2+, Ba2+, Fe3+, and
Co2+,while the inhibitor were Sn2+, Ca2+, Cu2+, Cd2+, Mn2+, Hg2+, and Ni2+. The
ions of Hg2+ andNi2+were founded stopped of the enzyme activity. The variables
of kinetic of amyloglucosidaewere obtained KM and vmaks value were. 4,40 % ,
0,49 mU, respectively.
Penulis: Amiruddin
Kode jurnal: jpkimiadd110190
