Keragaman Genetika Ramin [Gonystylus bancanus (Miq.) Kurz] dari Provinsi Riau Berdasarkan Profil Random Amplified Polymorphic DNA
ABSTRACT: Genetic Diversity of
Ramin [Gonystylus bancanus (Miq.) Kurz] from Riau Province Based onRandom
Amplified Polymorphic DNA Fingerprint. Gonystylus bancanus is a commercialtimber
found only on peat swamp forests, scatteredly distributed in Sumatra and
Kalimantan.Their existence is now under severe threat due to habitat
conversion. One of the remaining natural populations of ramin was in Riau
Province, Sumatra. This study aimed to assess genetic diversity of this species
within their natural populations in Riau Province using Random Amplified
Polymorphic DNA (RAPD). RAPD profiles were obtained by performing PCRamplification
using five arbitrary primers. One hundred and eleven putative loci of RAPD werescored
and analysed using Popgene and NTSYS software. Eleven of RAPD bands were commonly
found in all populations and 16 bands were distinctively found in certain
populations. These unique bands may serve as population diagnostic marker for
such populations. Theaverage genetic diversity within population (0.1606) was
lower than that of among populations (0.1894). Genetic differentiation (Gst)
indicated that 95.56% of total genetic diversity in raminwas attributed to the
differences among populations. The highest genetic diversity was foundin
population 3 (He:0.1858) and 3 (I:0.2864), while the lowest genetic variation
was observed inpopulation 1 (He: 0.1438) and 2 (I: 0.2201). Total genetic
diversity for all population (Ht) was0.1982 with an average value of genetic
diversity within populations (Hs) was 0.1606. The lowlevel of genetic diversity
found in ramin with high population differentiation may suggest that these remaining populations was undergoing
genetic bottleneck resulted from severe habitatfragmentation.
Author: Yulita Kusumadewi,
Yuyu S. Poerba, &Tukirin Partomihardjo
Kode Jurnal: jpbiologidd100196
