Karakterisasi Molekuler Ikan Gurami Soang (Osphronemus goramy Lac.) berbeda uuran menggunakan Pcr-Rflp Gen Sitokrom C Oksidase 1
Abstrak: Gourami (Osprhronemus
goramy Lac.) is a freshwater fish that
is widely cultivated in Indonesia,
particularly by fish farmer in Java. Soang strain has a faster growth rate than
other strains. However, the fry that derived from a single cohort (generated
from a single spawning) shows various sizes even in the same age because they
have different growth rate. Differences in growth rate may occur due to
differences in metabolic capabilities especially cellular respiration. Different
rate of respiration can be assumed to be due to differences in the genetic
component, especially on their cytochrome c oxidase 1 gene (CO1). Therefore
this study aimed to determine whether there are differences in PCR-RFLP marker
CO1 gene among different sizes of gourami soang strain from the same cohort so
that can be used to analyze genetic diversity. This study used survey method by
applying purposive random sampling. Four restriction enzymes were used during the
research. Molecular characters were defined descriptively based on the
appearance of restriction fragment on agarose gel. The result showed that soang
strain which used in this study had monomorphics allele on their CO1-HindIII.
PstI, BamHI, and EcoRI, could not cut the PCR products and results no RFLP
markers. So that genetic variation in the used CO1 gene fragment could not be
detected using HindIII, Pst1, BamH1 and EcoR1 enzymes. The three different
sizes of soang strain had similar alleles or homozygous, meaning that
heterozigocity value was 0 (zero). Therefore, the CO1 gene in this study could
not be used as molecular character to differentiate different sizes of gourami
soang strain from the same cohort.
Kata Kunci: gourami soang
strain, PCR-RFLP, genetic diversity, cohort, cytochrome c oxidase 1 gene (CO1)
Penulis: Siti Nur Azizah, Agus
Nuryanto, Hendro Pramono
Kode Jurnal: jpbiologidd150886
