ISOLATION AND IDENTIFICATION OF MOLD CONTAMINANTS ON MUSHROOM GROWING MEDIUM (BAG LOG) AND THEIR CELLULOLYTIC PERFORMANCE TEST
ABSTRACT: Mushrooms naturally
grows on logs that have experienced weathering. Raw materials of mushroom
growth media containing plenty of wild microbes, especially wild mold on
sawdust media. Sterilization conducted did not prevent the occurrence of
contamination. This study aim was to determine the types of contaminant molds
in cultivated mushroom growth media (bag logs), as well as determine their
cellulolytic performance. Mushroom growth media (bag log) samples were taken
from three mushroom cultivation locations. Mold isolates obtained were
identified by their macroscopic and microscopic characteristics. Test the mold
cellulolytic performance was conducted by measuring the ratio of hydrolysis
zone with the diameter of mold on CMC (Carboxy Methyl Cellullose) media. The
isolation and identification results of contaminant molds consisted of mold
isolates which could be grouped into 7 genus which were Aspergillus,
Penicillium, Paecillomyces, Trichoderma, Rhizopus, Fusarium and
Syncephalastrum. The cellulolytic performances of mold on CMC media showed that
Aspergillus flavus has the greatest cellulolytic ability.
Penulis: Tatik Handayani,
Susiana Purwantisari
Kode Jurnal: jpmatematikadd150846
