ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI PENGHASIL SENYAWA ANTIFUNGI TERHADAP Candida albicans
Abstract: Infectious diseases
in humans by microbes in developing countries including Indonesia remains a
major problem. Forty-three percent of deaths in developing countries are caused
by infections. Candida is a yeast species that often cause opportunistic infections.
Disease caused by Candida can affect the mouth, vagina, skin, nails, lungs,
sometimes can cause septicemia, endocarditis and meningitis. Rising
yeast-leavened which has resistance to antifungal compounds that exist,
encourage researchers continue to discover new compounds which have antifungal
properties. In addition, the side effects caused by the use of antifungal
drugs, causing people still expect the natural ingredients that can be used as
an alternative treatment. According to WHO, 80% of the world's population still
relies on traditional medicine, including the use of drugs from plants, one of
which is the use of turmeric plant (Curcuma zanthorriza). Bioactive compounds
of plants that are antifungal generally are volatile oil, aldehyde and phenol compounds.
The existence of endophytic microbes in plants have economic significance for
the industry favorable treatment. Endophytic microbes easily grown, have a
shorter life cycle than the plant and capable of producing bioactive compounds
similar to the host plant. Therefore, it is necessary to study the potential of
endophytic bacteria ginger rhizome (C. xanthorrhiza) as a producer of Candida
albicans antifungal compounds. This study uses ginger rhizome obtained from
Stone and Pasuruan. After the isolation of microbes from ginger rhizome,
further purification by means of subcultures. The endophytic microbial isolates
gram staining and identification of bacteria. The result is a gram-positive
bacterium Actinomyces viscosus, Bacillus brevis gram-positive bacteria and
gram-negative bacteria Pseudomonas stutzeri. Then do the production of
antifungal metabolites produced by endophytic bacteria and C. albicans
antifungal test Paper Disc Diffusion method. The result looks the inhibition
zone with a diameter of 4 mm, 15 mm and 45 mm
Penulis: Alvi Milliana, Wahyu
Safitri
Kode Jurnal: jpbiologidd150960
