Detection Limit Of Carbamate Pesticides Biosensors Based Enzyme Acetylcholinesterase And Cholin Oxidase On Platinum Electrode
Abstract: The presence of
carbamate pesticides in environmental and food poses a potentiahazard to human
health and there is a growing interest in their rapid and accurate
determinationfor food safety and environmental monitoring. The aim of this
research is to designelectrochemical biosensor for analyzingcarbamate
pesticides residue in food material.Acetylcholinesterase (AChE) and cholin
oxidise (ChO) enzyme was immobilized at platinum (Pt)wire plated with membrane
materials of SA 5%, 10%, 15% and GA 25%. Result of this researchshow that for
cellulose acetate (SA) 5% the detection limit is 10-7,7 M, for SA 10% the
detectionlimit is 10-8,7 M, for SA 15% the detection limit is 10-7,6 M. This a
results is approximately equal to 2,2 ppb). ), which means that this biosensor
is very sensitive for determining carbamatespesticides residue where its
detection limit is comparable to to the detection limit of conventionalinstrument
such as Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC),
i.e. respectively 1,5 ppb and 2,0 ppb.
Keywords: Biosensors,
immobilized, enzyme Acetylcholinesterase (AChE) and choline oxidase (ChO),
carbamate pesticides
Penulis: Mashuni
Kode Jurnal: jpkimiadd110193
