Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid
Abstract: bacteria that cause
an illness. Antibiotic treatments to a patient have a purpose to eliminate the
pathogen bacteria. Bacteria resistance to antibiotic was influenced by the
intensity of antibiotic treatment in a region, the uncontrolled antibiotics
treatments would increase the antibiotic resistance of bacteria. Plasmids was
an extrachromosomal DNA that encodes a functional protein that would eliminate
the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to
antibiotics. In this case it would be important to find out the bacterial
plasmid profile on the E.coli resistant to metronidazole and nalidixic acid
antibiotics. This research was using four different sample from faces of
diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli.
lasmid isolation method was carried out by lyses alkali method. Plasmid profile
of the E. coli that resistant to metronidazole and nalidixic acid antibiotics
and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was
observed as a fluorescent DNA band in ultraviolet rays. In result, isolated
plasmids from bacteria that resistant to antibiotics metronidazole and
nalidixic acid having similar size approximately 500 bp, different from
bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a
smaller size in region of 100 bp.
Penulis: Arya Iswara, Sri
Sinto Dewi
Kode Jurnal: jpbiologidd160454
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