ANTICANCER ACTIVITIES OF SECONDARY METABOLITES PRODUCED BY TAXUS ENDOPHYTIC FUNGUS PHOMOPSIS CHIMONANTHI
Abstract: Secondary metabolite
by the Taxus endophytic fungus strain Tse3 culture obtained from the West Java
Forest has been produced. Production of secondary metabolite had done by
aerobic fermentation for 7 days, 3(fC, 120 rpm in PDB (potato dextrose broth)
media. The broth of fermentation was extracted by ethyl acetate. Various
concentrations of the ethyl acetate extract in (0- 800) mcg/mL were prepared
and test against T47D cancer cell line. Cytotoxic test has be done by
MTTmethod. ICso value of ethyl acetate extract was 3 mcg/mL. The result of fungus
identification by morphology and molecular 18S rRNA was Phomopsis chimonanthi.
Penulis: A. Desak Gede Sri, J.
p. Raymond, Harmastmi Harmastini, L.B. S. Kardono,, M. Hanafi, E. Meiyanto
Kode Jurnal: jpkimiadd110181
