MONOSODIUM GLUTAMATE EXPOSURE AT EARLY DEVELOPMENTAL STAGE INCREASES APOPTOSIS AND STEREOTYPIC BEHAVIOR RISKS ON ZEBRAFISH (DANIO RERIO) LARVAE
Abstract: Excessive glutamate
may give neurotoxic effects and contribute to Autism spectrum disorder(ASD). In
this study, we investigated prolonged exposure effects of 10 µg/mL Monosodium
Glutamate (MSG) on intracellular calcium level, bax, bcl-2, ratio of bax/bcl-2
genes expression, caspase-3, apoptosis of brain cells and stereotypic behavior
of Zebrafish (Danio rerio) larvae at early developmental stages. Genes
expression were determined by real time PCR, caspase-3 using ELISA,
intracellular Ca2+ and apoptotic cells of brain using confocal microscopy,
locomotor activity by using crossing lines assay whereas stereotypic behavior
by circle swimming. The results indicated that MSG exposure increased brain bax
and bcl-2; and caspase-3; intracellular Ca2+; and apoptosis; stereotypic
behavior; and decreased locomotor activity. Termination of MSG treatments
resulted in recovery of bax, bcl-2, caspase-3 basal levels and stereotypic
behavior. In conclusion, MSG exposure at early embryonic stage increased brain
cell damage and risk of behavior changes.
Author: Nia Kurnianingsih
Journal Code: jpfarmasigg160034
