Immunomodulatory Compound from Myrmecodia pendens Merr & Perry
Abstract: Myrmecodia pendens
Merr & Perry (Rubiaceae) is widely used in Papua as traditional medicine against
various diseases. Preliminary study revealed that ethyl acetate fraction has
potency to increase mice Balb/c lymphocyte proliferation in vitro. This study
aimed to investigate the active compound responsible for the activity. Dried
powders of plant tubers were macerated in ethanol 95% followed by solvent
evaporation. The crude extract was further fractionated by liquid-liquid
partition with n-hexane, ethyl acetate and water, successively. The ethyl
acetate fraction was subjected to a silica gel column and separated by vacuum.
The subfraction yields were tested on mice Balb/c lymphocytes to observe the
influence on lymphocyte proliferation by MTT assay. Total phenolic content of
subfractions were determined by Folin-Ciocalteau method. Chemical
characteristics of active compound was identified by GC-MS, UV-Vis
spectroscopy, IR and H-NMR. The result showed that total phenolic content has no
signifcant correlation to the lymphocyte proliferation activity. Subfraction 7
yielded from ethylacetate-methanol (7:3v/v) contained a major constituent (78%
by GCMS, 83.75% by Densitometry). GCMS and UV spectra suggested a phenolic
substructure. IR and H-NMR suggested that the phenolic was glycosilated and had
aliphatic moiety.
Penulis: MARIA ULFAH, EDIATI
SASMITO, TRIANA HERTIANI
Kode Jurnal: jpfarmasidd130258
