Identification of Pathogenic Leptospira in Rat and Shrew Populations Using rpoB Gene and Its Spatial Distribution in Boyolali District
Abstract: Leptospirosis
becomes health problem in Indonesia. Until April 2014, cases of leptospirosis
transmitted by rats were reported in Boyolali with case fatality rate (CFR)
83.3%. Leptospira genus consists of various serovars and genetic types living
in different environment. Classification of Leptospira species based on rpoB
gene could be used as this gene has high level of polymorphism. This study
aimed to identify Leptospira serovars in rat population using kinship analysis
based on rpoB gene polymorphism and to describe spatial distribution of rats
with Leptospira positive in Boyolali District. A cross sectional study was
conducted on April 2014 at Sindon Village in Ngemplak Subdistrict and Jeron
Village in Nogosari Subdistrict, Boyolali District. Polymerase Chain Reaction
test was performed on 104 rat kidney samples from the two locations of study.
Spatial analysis was conducted to map distribution of rats with Leptospira
positive. There were six positive rpoB gene samples in Rattus tanezumi, Rattus
argentiventer and Suncus murinus. Five of six positive samples showed the
closest genetic kinship to Leptospira borgpetersenii serovar Sejroe based on
rpoB gene. One isolate did not have a close genetic kinship to any serovar
included in the cluster. Spatial analysis based on home range buffer zone
showed that rats with Leptospira positive were found in 30 meter and 150 meter
from leptospirosis patients.
Author: Dyah Widiastuti,
Zumrotus Sholichah, Agustiningsih, Nastiti Wijayanti
Journal Code: jpkesmasgg160021
