Typhonium flagelliforme decreases telomerase expression in HeLa cervical cancer cells
Abstract: Cancer cells have a
relatively high telomerase activity compared to normal cells, so that cancer
cells have the ability for continued proliferation and uncontrolled mitosis.
Telomerase is an enzyme responsible for the length of telomeres, DNA segments
located at the ends of eukaryotic chromosomes. Natural materials such as rodent
tuber (Typhonium flagelliforme) have anticancer potential. The purpose of the
present study was to determine the effects of Typhonium flagelliforme extract
on telomerase expression in HeLa cervical cancer and T47D breast cancer cells.
This experimental laboratory study was conducted on cultured HeLa and
T47D cancer cell lines, with normal Vero cells as controls, and using RPMI and
M199 culture media. The study comprised three groups, i.e. controls, and groups
receiving Typhonium flagelliforme extract at doses of ½ IC50 and IC50.
Telomerase expression was measured by immunohistochemistry (IHC). Analysis of
variance and LSD multiple comparison test were used to analyze the data.
Telomerase expression in cancer cells showed significantly higher values
compared to normal Vero cells. Typhonium flagelliforme extract was capable of
significantly decreasing telomerase expression in cancer cells receiving the
extract.
Typhonium flagelliforme extract at different doses is capable of
decreasing telomerase expression more effectively in cervical cancer cells than
in breast cancer cells. This study shows that Typhonium flagelliforme may have
anti-cancer activity, necessitating further investigations.
Author: Endang Purwaningsih,
Yulia Suciati, Etty Widayanti
Journal Code: jpkedokterangg160062