STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA
Abstract: Changes of sperm
heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were
investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C
for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to
identify sperm head morphometry, while for DNA integrity analysis using
acridine orange staining. Samples were smeared on glass slides, fixed for 2 h
in acetic alcohol and stained with acridine orange solution. After staining,
each slide was examined at x400 magnification in a fluorescence microscope with
axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green
emissions of the sperm head were examined and scored. These results indicated
that sperm head had enlarged significantly (P<0.05) after freeze-drying
process. However, freeze-dried sperm heads morphometry significantly
(P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA
integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after
incubation for 6 hours. In the present study concluded that (1) freeze-drying
spermatozoa caused sperm head morphometric enlarged, whereas incubation time
caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is
still intact during incubation 3 hours, and decreased DNA integrity occur in
incubation for 6 hours.
Author: S. Said, F. Afiati, T.
Maulana
Journal Code: jppeternakangg150055
