Serum TNF-α, IL-8, VEGF Levels in Helicobacter pylori Infection and Their Association with Degree of Gastritis
ABSTRACT: to investigate the
serum levels of TNF-α, IL-8, VEGF in Helicobacter pylori infection, and their association
with the degrees of gastritis histopathology. Methods: a cross-sectional study
was done on 80 consecutive gastritis patients admitted to endoscopy units at
Adam Malik General Hospital and Permata Bunda Hospital, Medan, Indonesia from
July-December 2014. The Rapid Urease test was used for the diagnosis of H.
pylori infection. The severity of chronic inflammation, neutrophil infltration,
atrophy, and intestinal metaplasia were assessed. Serum samples were obtained
to determine circulating TNF-α, IL-8, and VEGF. Univariate and bivariate
analysis (chi square, fsher’s exact, and mann-whitney test) were done using
SPSS version-22. Results: there were 41.25% of 80 patients infected with
Helicobacter pylori. Serum TNF-α and VEGF levels in the infected group were
signifcantly higher compared to H. pylori negative, but there were no signifcant
differences between serum levels of IL-8 in H. pylori positive and negative.
There were signifcant associations between serum level of TNF-α and IL-8 with
degree of chronic inflammation, and also between serum level of IL-8 and degree
of neutrophil infltration. There were signifcant associations between serum
level of VEGF and degree of atrophy, and also between serum level of VEGF and
degree of intestinal metaplasia. Conclusion: High levels of TNF-α were
associated with severe degree of chronic inflammation, high levels of IL-8
associated with severe degree of chronic inflammation and neutrophil
infltration, and high levels of VEGF associated with severe degree of
premalignant gastric lesion.
Key words: cytokine,
neoangiogenesis, Helicobacter pylori, atrophic gastritis, intestinal metaplasia
Author: Gontar A. Siregar,
Sahat Halim, Ricky R. Sitepu
Journal Code: jpkedokterangg150251
