Psidium guajava leaves decrease arthritic symptoms in adjuvant-induced arthritic rats
Abstract: Guava is an herbal
with proven antioxidant and anti-inflammatory properties. The aim of this study
was to investigate the anti-arthritic activity of the ethanol extract of
Psidium gujava leaves (EEPG) against complete Freund’s adjuvant (CFA) induced
arthritis in rats.
An experimental study was conducted on 40 male Wistar Sprague Dawley
rats, which were divided into 5 groups. Each group was induced with 0.2 mL CFA
(1 mg/mL) on day 1 and 0.1 CFA mL booster injection on day 5. Group I served as
an arthritic control, group II received dexamethasone (6.75 mg.kg-1 orally),
group III, IV and V received EEPG at oral doses of 250, 500, and 750 mg/kg BW,
respectively, on days 14 to 28. Anti-arthritic activity was observed from the
arthritis score, the paw circumference was measured on days 0, 1, 4, 8, 12, 16,
20, 24, and 28, the mobility score was determined on days 12 and 28, and the
histolopathology of the knee joint was examined on day 29.
Ethanol extract of Psidium guajava leaves significantly suppressed the
swelling of the paws in chronic phase based on increasing of edema (%), while
starting on day 20. EEPG at 250 mg/kg was most effective in significantly
reducing arthritis scores (p<0.05). Histopathological examination showed
repair of the knee joint synovial membrane and cartilage.
Psidium guajava leaf extract is effective in decreasing the inflammatory
response and arthritic symptoms in rats with adjuvant-induced arthritis.
Psidium guajava leaves can be developed into an alternative anti-arthritis
treatment.
Author: Hanif Nasiatul
Baroroh, Esti Dyah Utami, Anisyah Achmad
Journal Code: jpkedokterangg150286