Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Catle
ABSTRACT: Myostatin (MSTN)
gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy,
poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF
pathway, and glucolysis. Myostatin gene expression is controled by CpG island
located in promoter region. The objectives of this research were to identify polymorphism
of MSTN promoter gene and to associate the polymorphism of SNP with growth and
muscling traits in Bali catle. A total of 48 Bali catle from BPTU-HMT Bali
island was screened to identify genetic polymorphisms in MSTN promoter region using
sequencing method. The growth and muscling traits were measured at 12 months of
age. The muscling traits were evaluated using ultrasound console with linear
transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep.
Analysis of polymorphism was conducted by using PopGen 1.32 software. The
association of MSTN with growth and muscling traits were analyzed by using General
Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic
SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3
SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium
condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs
(g.-7799T>C and g.-7941C>T) were signifcantly associated with
intramuscular fat percentage (P≤0.05) in Bali catle. Although the 2 SNPs were
nominally signifcant at nominal P≤0.05 threshold, they were not signifcant
after Bonferroni correction for multiple testing. It could be concluded that
MSTN promoter gene was polymorphic in Bali catle and there were 2 SNPs
associated with carcass quality.
Author: H. Khasanah, A.
Gunawan, R. Priyanto, M. F. Ulum, & Jakaria
Journal Code: jppeternakangg160004
