Effect of 1.2-epoxy-3[3-3[3.4-dimetoxyiphenil]-4h-1-benzopiran-4-on] Propane (epi) on Sirtuin-1 and Nuclear Factor-kB Expression of DMBA induced Mammary Tumors in sprague dawley rats
Abstract: The main factors
contribute in breast cancer development is the combination of exogenous and
endogenous factors. Endogenous factors include both SIRT1 and NF-κB. Exogenous
factor used in this research is DMBA.
1,2-epoxy-3[3-3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) is a
derivative of isoflavone generate from clove leaf oil.
Objectives: To examine the effect of EPI on SIRT1 and NF-κB expression in
DMBA-induced Sprague Dawley rats, and to examine the correlation of SIRT1.
Methods: This experimental study with post-test only group design used 35
Sprague Dawley female rats with 2 weeks old. Those rats were divided into 7
groups (5 rats/group), namely normal control (1st group), corn oil group (2nd
group), DMBA group (3rd group), EPI treated groups with 1 mg/kgBW, 2 mg/kgBW,
and 4 mg/kgBW, respectively (4th, 5th, and 6th groups), and doxorubicin group
(7th group). EPI and doxorubicin were given from 1st until 15th week, while
DMBA were administered from 3rd until 9th week. At the end of week 15th, all
rats were terminated and all of breast organs of each rats were taken to be
prepared as paraffin block samples. Examination of SIRT1 and NF-κB expression
was performed using light microscope at 400x magnification, after
immunohistochemistry (IHC) staining. Expression of SIRT1 and NF-κB were
analyzed using IHC-profiler plug-in in ImageJ software.
Results: Both SIRT1 and NF-κB expression in SD rat groups were given EPI
has lower than DMBA group (p=0,000), and found there was no significant
differences with Doxorubicin group. Furthermore, we found that there is a positive
correlation between both SIRT1 and NF-κB expression (p=0,001; r=0,773) in
EPI-treated SD that were induced by DMBA
Conclusions: EPI was able to prevent an increasing of SIRT1 and NF-κB
expression in SD model breast cancer that induced with DMBA. There is a
positive correlation between SIRT1 and NF-κB expression in EPI-treated SD rats
that were induced by DMBA.
Keywords: Isoflavone, SIRT1
expression, NF-κB expression, breast cancer, EPI, DMBA, Sprague Dawley rats
Author: Ayyub Harly Nurung,
Sri Herwiyanti, Dewi Kartikawati Paramita
Journal Code: jpkedokterangg160186
