Detection of Carbapenemase Encoding Genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii Isolated from Patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011
ABSTRACT: to determine the
prevalence of carbapenemase encoding genes (blaIMP-1, blaVIM-2, blaKPC-2, blaOXA-48,
and blaNDM-1) of carbapenem-resistant Enterobacteriaceae, Pseudomonas
aeruginosa and Acinetobacter baumanii isolated from the intensive care unit
patients as pathogens, in Cipto Mangunkusumo hospital (ICU-RSCM) in 2011.
Methods: we examined the carbapenemase encoding genes in the clinical
microbiology laboratory (LMK FKUI/RSCM). Duplex- and simplex PCR methods were
conducted to detect the resistant genes. Results: we found 4 (5%) P. aeruginosa
strains carry blaIMP-1 gene and all were isolated from sputum specimens. The
prevalence of carbapenem resistant among Gram-negative bacilli isolated from
ICU-RSCM, are Enterobacteriaceae 27.6%, P. aeruginosa 21.9%, and A. baumannii
50.5%. The New Delhi Metallo-β-lactamase encoding gene (blaNDM-1) was detected
in 1 K. pneumonia isolated from sputum as well. The other genes, i.e. blaKPC-2,
blaVIM-2, and blaOXA-48 were not found in any isolates. The absence of other
genes indicated that other mechanisms may play a role in the occurrence of
carbapenem resistance in pathogens isolated in ICURSCM. Conclusion: this study
confrmed that the prevalence of carbapenems resistant Gram-negative bacilli in ICU-RSCM
in 2011 was high. The carbapenemase encoding genes, which were detected among
the carbapenems resistant Gram-negative bacilli, were blaIMP-1 and blaNDM-1.
Author: Anis Karuniawati,
Yulia R. Saharman, Delly C. Lestari
Journal Code: jpkedokterangg130257
