Black sugarcane decoction reduces rat brain ischemia
Abstract: There are people in
Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD
contains policosanol and antioxidants. It has been proven that policosanol can
reduce global ischemia in Mongolian gerbils. This study aims to evaluate the
effect of BSD on brain ischemia in a rat stroke model.
A laboratory experiment using eighteen 3-month old male Wistar rats
without any defects, of 175-250 g body weight. Brain ischemia was produced by a
20-minute bilateral carotid communis artery oclusion (BCCAO). Using a rat stroke model, brain ischemia was produced
by a 20-minute BCCAO. The rats were randomized into three groups: BSD treated
stroke model rats (group 1), non treated stroke model rats (group 2), and sham
operated rats (group 3). BSD was administered by gavage for 1 week before
BCCAO. Decapitation of rats was performed two hours post BCCAO. Brain tissues
were stained with 2,3,5-triphenyltetrazolium chloride (TTC). Ischemic areas
were analyzed using Image J softwere. Statistical analysis was conducted by one
way ANOVA test.
The mean percentages of rat brain ischemic area differed between group 3
(0.0 ± 0.0%), group 2 (3.13 ± 0.59%) and group 1 (1.15 ± 0.47%) p =0.001). Post
hoc test showed that there was no difference between group 3 with group 1.
Instead, there was a significant difference between group 2 and the other groups.
The administration of BSD reduced rat brain ischemia after bilateral
carotid artery ligation.
Author: Ety Sari Handayani,
Zainuri Sabta Nugraha, Titis Nurmasitoh, Kuswati Kuswati, Dwi N. Ahsani, Ajeng
G. Nanda
Journal Code: jpkedokterangg160041
