Bioactive Compounds Isolated from Lignin of Empty Bunch Palm Fiber and Their Effects on in Vitro Rumen Fermentation
Abstract: The objective of
this experiment was to study the effects of bioactive compounds isolated from
purified lignin formacell (PLF) of empty bunch palm fiber as natural
antimicrobes and their effects on in vitro rumen fermentation. The first
experiment was inhibition test of 11 bioactive compounds isolated from PLF,
using disc diffusion method against the growth of Escherichia coli, Salmonella
typhimurium, and Staphylococcus aureus. Four of the most potential bioactive
compounds were then used in the second experiment, which was an in vitro test
using fresh rumen liquid of Ongole grade beef cattle, to study their effects on
rumen fermentation. Six treatments with 3 replications were applied in a
completely randomized block (CRB) design. The treatments were R0= 0.5 g basal
diet; R1= R0 + 0.3 mg Rumensin®; R2= R0 + 0.3 mg syringaldehyde; R3= R0 + 0.3
mg p-hydroxybenzoic acid; R4= R0 + 0.3 mg m-hydroxybenzoic acid; and R5= R0 +
0.3 mg oxybenzene. Isolate fraction of CC-2 (syringaldehyde), CC-3
(m-hydroxybenzoic acid), VLC-5 (oxybenzene), and VLC-9 (p-hydroxybenzoic acid)
exhibited antimicrobes activity against all tested bacteria. Other isolated
fractions exhibited antimicrobes activity only against 1 or 2 tested bacteria.
The use of syringaldehyde, p-hydroxybenzoic acid, m-hydroxybenzoic acid, and
oxybenzene improved ammonia concentration, microbial protein synthesis, and
nutrients digestibility. Bioactive compounds had no effect on rumen pH but
reduced total VFA concentration as well as the estimate of methane production.
Author: M. Prayuwidayati, T.
C. Sunarti, . Sumardi, . Subeki, K. G. Wiryawan
Journal Code: jppeternakangg150051