ASSOCIATIONS BETWEEN POLYMORPHISM OF GROWTH HORMONE GENE WITH MILK PRODUCTION, FAT AND PROTEIN CONTENT IN FRIESIAN HOLSTEIN CATTLE
Abstract: The aim of the
research was to determine the associations between polymorphism of the bovine
growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein
Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups
(from New Zealand=19 heads and Australia=43 heads) were used for the research.
We performed the Polymerase Chain Reaction and followed by Restriction Fragment
Length Polymorphism using AluI restriction enzyme. The genotype was observed
base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth
hormone gene. The frequencies of genotypes LL were found higher than genotype
LV in both groups. Friesian Holstein cattle from New Zealand showed the
genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from
Australia show the genotype LL and LV as 79% and 21%, respectively. The
association between Leu/Val polymorphism on milk production, fat and protein
content in both groups did not show the significant effect. Base on two groups
of the origin of cattle, the result showed the significant different on fat and
protein content of milk. Fat and protein contents of milk were higher in breed
of FH imported from Australia compared to those in breed of FH imported from
New Zailand.
Author: T. Hartatik, D.
Kurniawati, A. Adiarto
Journal Code: jppeternakangg150053
