Antifungal Susceptibility Testing in HIV/AIDS Patients: a Comparison Between Automated Machine and Manual Method
ABSTRACT: to evaluate the
performance of Vitek2 compact machine (Biomerieux Inc. ver 04.02, France) in
reference to manual methods for susceptibility test for Candida resistance
among HIV/AIDS patients. Methods: a comparison study to evaluate Vitek2 compact
machine (Biomerieux Inc. ver 04.02, France) in reference to manual methods for
susceptibility test for Candida resistance among HIV/AIDS patient was done. Categorical
agreement between manual disc diffusion and Vitek2 machine was calculated using
predefned criteria. Time to susceptibility result for automated and manual
methods were measured. Results: there were 137 Candida isolates comprising
eight Candida species with C.albicans and C. glabrata as the frst (56.2%) and
second (15.3%) most common species, respectively. For fluconazole drug, among
the C. albicans, 2.6% was found resistant on manual disc diffusion methods and
no resistant was determined by Vitek2 machine; whereas 100% C. krusei was
identifed as resistant on both methods. Resistant patterns for C. glabrata to
fluconazole, voriconazole and amphotericin B were 52.4%, 23.8%, 23.8% vs. 9.5%,
9.5%, 4.8% respectively between manual diffusion disc methods and Vitek2
machine. Time to susceptibility result for automated methods compared to Vitex2
machine was shorter for all Candida species. Conclusion: there is a good
categorical agreement between manual disc diffusion and Vitek2 machine, except
for C. glabrata for measuring the antifungal resistant. Time to susceptibility
result for automated methods is shorter for all Candida species.
Author: Erni J. Nelwan, Evi
Indrasanti, Robert Sinto, Farida Nurchaida, Rustadi Sosrosumihardjo
Journal Code: jpkedokterangg160012
