Pigments Characterization and Molecular Identification of Bacterial Symbionts of Brown Algae Padinasp. Collected from Karimunjawa Island
Abstract: The search for
carotenoids in nature has been extensively studied because of their
applications in foods. One treasure of the biopigment source is
symbiotic-microorganisms with marine biota. The advantages of symbiont bacteria
are easy to culture and sensitize pigments. The use of symbiont bacteria helps
to conserve fish, coral reefs, seagrass, and seaweed. Therefore, the bacteria
keeps their existence in their ecosystems. In this study, bacterial symbionts
were successfully isolated from brown algae Padina sp. The bacterial symbionts
had yellow pigment associated with carotenoids. The pigments were characterized
using High Performance Liquid Chromatography (HPLC) with a Photo Diode Array
(PDA) detector. The carotenoid pigments in the bacterial symbionts were
identified as dinoxanthin, lutein and neoxanthin. Molecular identification by
using a 16S rRNA gene sequence method, reveals that the bacterial symbionts
were closely related to Bacillus marisflavi with a homology of 99%.
Author: Damar Bayu Murti, A B
Susanto, Ocky Karna Radjasa, Ferdy Semuel Rondonuwu
Journal Code: jpperikanangg160023
