PEMERIKSAAN Viral Nervous Necrosis (VNN) PADA IKAN DENGAN METODE Polymerase Chain Reaction (PCR)
Abstract: Fish production
annually has increased. Trading activities of Fishery in Indonesia both exports
and imports will continue to increase every year. Control and monitoring of
fisheries products was transported in Indonesia aims to reduce the risk of
entry and spread of pests and diseases of fish in Indonesia. The purpose of
Study is to know examination techniques of virus Viral Nervous Necrosis (VNN)
at fish by Polymerase Chain Reaction (PCR) method. This Study was held in the
Quarantine Fish Quality Control and Safety of Fishery Products (BBKIPM-KHP)
Soekarno Hatta Tangerang, Banten on January 12 th to February 12 th , 2015. The
methods of data fetching consisted of primary and secondary data. The data
fetching was performed with active participation, observation, interviews, and
literature. Examination methods Viral Nervous Necrosis (VNN) by Polymerase
Chain Reaction (PCR). Stages of virus examination by PCR method includes
necropsy samples, RNA extraction, amplification, electrophoresis, and the
diagnosis results using UV transluminator. Fish samples were examined VNN virus
consisting of a live fish and frozen fish both commodity exports, imports, or
domestic. The results showed that all fish samples are negative infected with
the virus VNN.
Penulis: Elly Fitriatin dan
Abdul Manan
Kode Jurnal: jpperikanandd150162
