Oil Spill Biodegradation by Bacteria Isolated from Jakarta Bay Marine Sediments
Abstract: A laboratory study
was conducted with the aim to isolate and identify bacteria from sea sediment
and test their biodegradation ability in two place where contaminated with oil
spill. Five sediment samples were dissolved by using sterile sea water, and
then bacteria isolated with total plate count (TPC) method. Isolates bacteria
was cultivated, and adapted using the nutrient conditioned sea water medium.
Biodegradation process was done by mixing the bacteria with crude oil and
shaken for few days. The number of bacteria isolated varied from 2 x 102 CFU
ml-1 to 6 x 106 CFU ml-1 and apparently increased after cultivation and
adaptation with oily media. Bacteria identified during this study were
Fundibacter sp., Alcanivorax sp., and Marinobacter sp.. The result of
biodegradation process was statistically analyzed and obtained that the
bacteria are effective in degrading oil in seven days with constant of
biodegradation rate was 0.1766. GC-MS analysis was conducted to prove the
decomposition of carbon chain by bacteria and revealed oil degradation in
carbon number 11 to 27. Based on all analysis, marine sediment bacteria can
degrade the oil spill.
Penulis: Angga Dwinovantyo,
Tri Prartono, Restya Rahmaniar, Nico Wantona Prabowo, Santi Susanti
Journal Code: jpperikanangg160019
