Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract
Abstract: Digestive tract is
complex environment consist of large amount of bacteria’s species. Fish
intestine bacteria consist of aerobic or facultative anaerob bacteria which can
produce antibacterial and enzym. The objectives of this research were to
isolated lactic acid bacteria that produce bacteriocin-like and protease from
mud crab digestive tract. Isolation and characterization of isolates were
conducted employing media MRS.
Neutralized cell free supernatant of isolates were tested using disc
diffusion agar of against pathogenic and spoilage bacteria to indicate
bacteriocin-like-producing lactic acid bacteria. Protease-producing isolate was
tested using disc diffusion method in casein agar. Among a hundred isolates, 96
isolates were showed clear zone in MRS+CaCO3,, catalase negative, and Gram
positive bacteria. Thirty four isolates produced protease and only four
isolates (i.e. IKP29, IKP30, IKP52, and IKP94) showed strong inhibition against
pathogenic and spoilage bacteria. There were three patterns of inhibition among
three isolates against Bacillus subtilis, Staphylococcus aureus, Eschericia
coli, and Salmonella sp. All three isolates showed potential uses for produce starter
culture for fishery product fermentation purpose. This is the first report of
isolation lactic acid bacteria that produced protease and bacteriocin-like from
digestive tract of mud crab.
Author: Heru Pramono, Pipin
Suciati, Sapto Andriyono
Journal Code: jpperikanangg150009
