DETEKSI DISTRIBUSI WHITE SPOT SYNDROME VIRUS PADA BERBAGAI ORGAN UDANG VANAME (Litopenaeus vannamei)
ABSTRACT: One way to detect
the presence of the White Spot Syndrome Virus in several organs Vaname shrimp (Litopenaeus
vannamei) were infected with the method of Polymerase Chain Reaction (PCR).
WSSV virus is known to attack various target organs, but it is difficult to
detect early on shrimp. Therefore, this study aims to detect the presence of
the distribution of White Spot Syndrome Virus in several organs Vaname shrimp (Litopenaeus
vannamei) were infected with the Polymerase Chain Reaction method in order to
early detection efforts for the prevention and treatment of diseases caused by
the virus. Organ observed that the swimming legs, gills, stomach and antennal
gland. Research stages include sample preparation and DNA extraction, DNA amplification
and electrophoresis. DNA extraction was performed using the method DTAB and
CTAB-DNA amplification is done by using nested PCR. The emergence of DNA bands
is an indicator of the presence of WSSV in shrimp Vaname several organs which
are then presented desktriptif. The results showed four WSSV infected organs
were observed. Based on the appearance, the entire sample of the swimming leg
and antennal gland positively infected, then successively gills and stomach.
The swimming leg can be used to diagnose the disease early on shrimp WSSV
Vaname without turning off the shrimp.
Keywords: Distribution,
Vanname shrimp, WSSV, PCR
Penulis: A.Aliah Hidayani , Asmi C. Malina, Bunga R. Tampangallo, dan Achmad F.
Fathurrahman
Kode Jurnal: jpperikanandd150157
