AKTIVITAS ANTIKANKER PROTEIN KAPANG Xylaria psidii KT30
ABSTRACT: Previous research
shows that protein of an algicolous fungus Xylaria psidii KT30 inhibited HeLa
cell with an average 264.7 μg/mL. To enhance the potent anticancer activity of
extracellular protein from Xylaria psidii KT30, the present research
demonstrated purification of its secreted protein. Anticancer against Chang
cell line (liver cell) and HeLa cell line (cervical cancer) was done by MTT
cytotoxic method. Protein concentration assay was done by bicinchoninic acid
(BCA) protein assay kit. Anticancer assay showed protein of fungus KT30 was not
toxic against Chang cell line and HeLa cell line. Based on calculation, IC50
value protein of fraction ammonium sulfat 90% and fractions 11-12 were 670.86
and 1451.68 µg/mL. The protein concentration of fraction ammonium sulfat 90% and
fractions 11-12 were 9.013 and 0.604 mg/mL.
Penulis: Aris Munandar, A.
Zaenal Mustopa, Kustiariyah Tarman, Tati Nurhayati
Kode Jurnal: jpperikanandd150197
