VALIDASI METODA PENENTUAN ANTIOKSIDAN TOTAL (DIHITUNG SEBAGAI ASAM SITRAT) DALAM SAMPEL JERUK SECARA SPEKTOFOTOMETRI DENGAN MENGGUNAKAN OKSIDATOR FeCl3 DAN PENGOMPLEKS ORTO-FENANTROLIN
ABSTRACT: Antioxidant is
a compound that
can be used
to obstruct species
oxygen reactive, species nitrogen, and other free radicals so
it can be used degenerative illnesses inhibition. Citric acid is a natural
antioxidant that can be found in some
kind of oranges. There are some methode that usually used to
determination the antioxidant, such as CUPRAC, DPPH, and FRAP. Objective of
this research is studied about alternative method to determine total
antioxidant that is counted as
citric acid by
using FeCl3 as
an oxidator and
o-phenantroline as a
complexant reagent. In studied
of appropriateness of
this method, validation
has been done
with parameter linearity, LoD, LoQ, SDR, and recovery with
samples orange, kaffir lime, and lime. Base on the research, the regression
equation is Y = 0,066 + 117.35 X and R2 = 0.990 with value of LoD is1.15x10-3 M
and LoQ is 3.86x10-3 M. The value of SDR and recovery in samples orange, kaffir
lime, and lime are 1.64%, 0.53% 2.14%, and 100.47%, 93.58%, 93.78%,
respectively. Base on validation method data, can be concluded that this method
appropriate to be used to determination of total antioxidant for samples
orange, kaffir lime, and lime.
Penulis: Yefrida, Mega
Ulfaningsih, Umiati Loekman
Kode Jurnal: jpkimiadd140713
