UJI TOKSISITAS DAN AKTIVITAS ANTIBAKTERI EKSTRAK DAUN MERAH TANAMAN PUCUK MERAH (Syzygium myrtifolium Walp.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli
Abstrak: A research aims to
know the toxicity of Pucuk Merah (Syzygium myrtifolium Walp.) red leaves
extracts against brine shrimp Artemia salina Leach and its bioactivity against
Escherichia coli and Staphylococcus aureus has been carried out. The dried
samples were macerated with ethanol and were fractionated with n-hexane, ethyl
acetate and ethanol-water. Phytochemical test showed the presence of alkaloids,
triterpenoids, steroids, saponins, phenolic and flavonoid in ethanol extract.
n-Hexane fraction contained alkaloids, triterpenoids and steroids, ethyl
acetate fraction contained alkaloids, triterpenoids, phenolic and flavonoid
while ethanol-water fraction contained triterpenoids, saponins and phenolic.
Brine Shrimp Lethality Test showed that ethyl acetate fraction had the highest
bioactivity in Artemia salina Leach with LC50 values of 149.8600 ppm.
Antibacterial activity test using agar diffusion method showed that ethyl
acetate fraction had the highest inhibiton against Staphylococcus aureus
whereas total extract had the highest inhibition against Escherichia coli with
MIC values of 0,5%. These results showed that Syzygium myrtifolium Walp. red
leaves extract potential as an antibacterial agent.
Penulis: Nur Aini Haryati,
Chairul Saleh, Erwin
Kode Jurnal: jpkimiadd150428
