TRANSFORMASI Agrobakterium rhizogenese DAN INDUKSI AKAR RAMBUT PADA TANAMAN KAKAO (Theobromacacao) UNTUK PRODUKSI SENYAWA ANTIOKSIDAN SECARA INVITRO
ABSTRACT: Transformation of Ri
T-DNA Plasmid Agrobacterium rhizogenese to varieties Theobroma cacao variety
TSH which is growing in west Sumatra and induction of hairy roots in order to
produce bioflavonoid antioxidant compounds such as, catechin, polyfenol, or
monomer and oligomer flavones was successfully obtained. Three spesies of
A.rhizogenese (A4,LBA 9457 and ATTCC 15834) originaly from LIPI was used to
transform Ri T-DNA plasmid in MS medium via cacao embryo culture. The aim of
this paper is to determine the affectivity and ability of the three species of
bacterial above to produce hairy roots in cacao invitro culture. The
statistical methods RAL was uses with 4 time treatments and 6 time repeated experiments.
As treatment was bacterial inoculation and without inoculation as a control. The
transformation result shows 2 of 3 of bacterial species have ability to induce
hairy roots of.T cacao embryos counting by percentages of explants with
producing hairy roots 16.66% for A4, 83.33% for LBA 9547 spesies.qualitative
test of polyfenol from hairy roots transformants give (+4) as compared to non
transform only (+1). Cathechin compound was determined by spectrophotometer as
much as 0.1% for non transform and 0.87 % for hairy roots transformants by LBA
9547. Conformation of plasmid Ri T-DNA hairy roots from two transformants was
analysis by PCR methods. The two primers rol B1 (52ATGGATCCCAAATTGCTTCCCCCACGA32)
dan rol B2 (53 TTAGG CTTTCATTCGGGTTTACTGCAGC 33) was used. For TR-DNA the
primes used is TRI (53 GGAAATTGTGGCGTTGTTGTGGAC 3’) and TR2 (5’ AATCGTTCAGAGAGCGTCCGA
AGTT 3’) . PCR analysis of DNA electrophoresis founded the band of TL region at
780 bp and TR at 1600 bp using DNA Ledder as DNA standard.
Keywords: transformation
A.rhizogenese, PCR, Theobroma cacao, kultur embrio, kultur akar rambut,
metabolit sekunder, cathechin
Penulis: Sumaryati Syukur dan
Zozy Aneloi N dan Femilya Putri
Kode Jurnal: jpkimiadd090225
