Profile of T cells after dexamethasone treatment in BABL/c mice
ABSTRACT: Regulation of
homeostatic mechanisms and the development of T lymphocytes is influenced by several
things, one of them is glucocorticoids. Dexamethasone is a synthetic
glucocorticoid drugs used in the treatment for several diseases acted as an
anti-inflammatory. The aim of this experiment was to confirm the effect of
dexamethasone at normal dose and high dose on T cell profile and also to know
the quantity of T cells after dexamethasone injection in each dose. This
experiment we applied 18 mice in the age of 2 week and divided into 3 treatment
groups with six replication i.e. control, dexamethasone injection with normal
dose (0.5 mg/kg BW) and high dose (10 mg/kg BW), then observed on day-7 after injection.
T cells were isolated from the spleen and analyzed by flow cytometry. Data
analysis was confirmed with the ANOVA test followed by Turkey test with
significance different (α) of 0.05. The result showed that dexamethasone act as
immunosuppressant agent on high dose (10 mg/kgBB). Dexamethasone injection with
normal dose on healthy mice showed no significant different in total number of
CD4 + , CD8 + , CD4 + CD62L, and CD8 + CD62L compared to control. But,
dexamethasone injections with high dose showed that the total number of CD4 + ,
CD8 + , CD4 + CD62L, and CD8 + CD62L were decreased significantly.
Author: Iga Dwi Januarisasi,
Muhaimin Rifa’i
Journal Code: jpbiologigg150006
