Profil Reproduksi Jantan Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Stadia Muda, Pradewasa, dan Dewasa
ABSTRACT: Reproductive biology
is one of prominent studies in biomedical research. Disruption in reproductive
system becomes a major problem both in humans and animals. Preclinical study
using animal model is therefore needed to initiate clinical studies and
diagnostic purposes. Wistar rats are commonly used for research in male and
female reproductive system due to their representation of mammal biological
system. For that purpose, their reproductive age must be determined to meet the
aim of studies. This research was carried out to provide reproductive profile
of normal male Wistar rats from different ages, furthermore categorizing them
into three checkpoints: young, subadult, and adult. Variables observed
including: body mass, testosterone level, reproductive glands index, spermatogenesis,
and sperm analysis. Results demonstrated that body mass, testosterone level,
and reproductive glands index increase with age (significant at the age of 6–7
weeks). Spermatogenesis is initiated at the age of 7 weeks, characterized by
significant increase in the number of spermatogenic cells which then maintained
at subsequent ages. Spermatozoa has been produced at the age of 6 weeks, however
still in low concentration, immotile, and not viable. The quantity and quality
of sperm also increase with age. At the age of 8–9 weeks, sperm concentration
significantly increases, progressive movement occurs, and viability is close to
100 %. In conclusion, rats aged 4–5 weeks can be categorized as young, sexually
immature; rats aged 6–7 weeks are subadult, the reproductive system has
well-developed (puberty) but spermatozoa are still immotile (infertile); rats
aged 8–9 weeks are adult, sexually mature, and ready for mating, thus suitable
as animal model for the study of reproductive system.
Penulis: LAKSMINDRA FITRIA,
MULYATI, CUT M. TIRAYA DAN ANDREAS S. BUDI
Kode Jurnal: jpbiologidd150614
