PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK
ABSTRAK: Protease alkaline and
keratinase are a group of protease enzym which have important value in detergen
industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria
isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to
produce protease and keratinase. The purpose of this research is to get
protease alkaline and thermostable keratinase from Brevibacilus agri-A03.
thermostable enzym is produced from enzym production enzym that contains kasein
and keratin at various medium pH, inoculum incubation temperature and medium
type. Enzym activity is measured by modified Walker methode, protein content is
measured by Lowry methode. Protease alkaline is produced at exponential phase,
maximum at 18th hours of incubation and keratinase is produced at stationer
phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH
condition 9.0; incubation temperature 55°C, inoculum 5% by using modified
Johnvesly and Naik medium with each protease and keratinase specific activity
1.927 and 1.047 U/mg
Penulis: Anthoni Agustien,
Jetty Nurhajati, Linar Z. Udin, Pingkan Aditiawati
Kode Jurnal: jpkimiadd100177
