PRODUKSI ENZIM AMILOLITIK DARI BACILLUS MEGATERIUM MENGGUNAKAN VARIASI KADAR PATI SAGU (METROXYLON SP.)
ABSTRACT: The application of
enzymes as biocatalysts for the industries in Indonesia has increased. Among
the enzymes that are needed in Indonesia, one of which amylolytic enzymes.
Amylolytic enzymes constitute a group of enzymes that catalyze the hydrolysis
of starch into simple sugars consisting of glucose units. In this study
amylolytic enzyme isolated from the bacterium Bacillus megaterium. Enzyme production
was submerged fermentation method for 14 hours using sagostarch Metroxylon sp.
varies. Measurement of enzyme activity was determined by the method amylolytik
Somogy-Nelson. Research results showed that2 % (w/v) of sago starchis the
optimum consentration in media with highest amylolytic enzyme activity that is
equal to 0.076 units/ml and sugar medium formed by181.254ug/ml in the
fermentation medium.
Penulis: Sandra Madonna
Kode Jurnal: jpbiologidd141012
