Isolasi dan Uji Efektivitas Beberapa Isolat Dekomposer Lokal dalam Mendekomposisi Limbah Tanaman Jagung
Abstrak: Soil microorganisms
are able to digest and degrade organic material present in the environment in
to substances that available for plants to absorb. This research aimed to
isolate and examine local bacteria that had the ability to decompose corn
stover. Soil samples were taken from various natural habitats in which bacteria
grow, including remains of corn planting area, pile of waste corncob and corn
stalk, pile of rice husk and straw, and pile of used sorghum stalk. Which were
collected from several locations across South Sulawesi. Bacteria was isolated
by making soil suspension that being inoculated on to NA and CMC medium, and
then incubated for 48 hours at room temperature. Bacterial colonies that grow
were then incubated 50o C to obtain bacteria resistant to high temperature and
able to produce cellulose. Bacterial isolates that produce cellulose was then
examined for the effectiveness in decomposing corn stover waste. Isolates
result were able to obtain 119 decomposers isolates with 29 isolates resistant
to temperature above 50o C and had ability to produce cellulose. From 29
isolates, among 15 isolates collected from rice straw, 5 isolates from remains
of corn planting area and 9 isolates collected from several natural habitats. Almost
all isolates had good ability to decompose corn stover waste. From isolates
tested in corn cob waste and corn leaves waste, only 16 bacterial isolates were
proven to be effective as decomposer.
Penulis: Nurasiah Djaenuddin,
Faesal Faesal, Soenartiningsih
Kode Jurnal: jpbiologidd140932
