ISOLASI DAN KARAKTERISASI SENYAWA STEROID PADA FRAKSI N-HEKSANA DARI DAUN KUKANG (Lepisanthes amoena (HASSK.) LEENH.)
Abstrak: Kukang (Lepisanthes
amoena (Hassk) Leenh.) Leaves is one of the species that belonging to the
Sapindaceae family. Traditionally used as drug ulcerate and skin care and also
known it has potency of tironase inhibitor and antioxidant agent, but compound
that contained in that plant was unknown. The purpose of this research was for
isolating steroid compound of n-hexane fraction from Kukang (Lepisanthes amoena
(Hassk) Leenh.) leaves and characterizing steroid compound with phytochemical
screening, R f , melting point and IR spectrum. Extraction of 650 grams powder
Kukang (Lepisanthes amoena (Hassk) Leenh.) leaves with methanol yielded 121,82
grams of crude methanol extract. The crude methanol extract was fractionated
with n-hexane and obtained 2,32 grams of n-hexane fraction. Results of
phytochemical screening showed that the n-hexane gave positive result for
steroid compound. Separation of steroid compound in n-hexane fraction by column
chromatography with silica gel 60 (70-230 mesh) which eluted using n-hexane :
ethyl acetate (8 : 2) eluent based on Isocratic method and gave nine fractions.
Phytochemical screening by Liebermann Burchard reagent showed F fraction
contained steroid compound and have been crystalized needle. Purification by
recrystallization of the F fraction gave the needle white crystal 10,2 mg with
reterdation factor (R f ) n-hexane : CHCl 3 (1 : 1) = 0,40 ; n-heksana : CHCl 3
(3 : 7) = 0,50 CHCl 3 (100%) = 0,71 ; n-hexane : EtOAc (3 : 7) = 0,82 ; EtOAc
(100%) = 0,86. Isolated compound has melting point 143-144°C, IR λmax (cm -1 ):
960,48; 1056,92; 1380,94; 1461,94; 1639,38; 2866,02; 2935,46; 3433,06. Based on
its physical and spectroscopic characterization, the isolated compound is
supposed as sterol of steroid compound.
Penulis: M.Riza Agus Pramana,
Chairul Saleh
Kode Jurnal: jpkimiadd130720
