Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura
Abstract: The study about the
activity of the enzyme amylase from amylolytic bacterial isolates from wet
sagoo starch and its traditional provision environment had been done in
Jayapura. The purposes of this study were to determine the activity of amylase
enzyme and to identify the bacteria isolated from wet sagoo starch and its
processing environment in Jayapura district. The method used was an
experimental laboratorium in which isolation of amylolytic bacteria was
performed by using nutrient agar medium with 1% soluble starch on spreed pour plate
method. The enzyme activity was detected with 0.2% iodine in 2% potassium
iodide which were able to form a clear zone. The protein content of the crude
enzyme extract was determined by the Bradford method using bovine serum albumin
(BSA). Amylase enzyme activity was determined by the formula: DUN/ml =
[(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results
showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial
isolates have amylolytic power of more than 30 mm. The amilase activity of
amylolytic bacterial of all isolates were quite high: which were 35 577, 18
903, 32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The
identification of isolates indicated that the three isolates are members of the
Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%,
and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value
of 94.30%.
Penulis: Suprapto, Tri
Gunaedi, Basa T. Rumahorbo
Kode Jurnal: jpbiologidd140922
