Studi Agronomis Tanaman Padi (Oryza sativa L.) Hasil Ko-Kultivasi Beberapa Strain Agrobacterium tumefaciens
Abstrak: The transgenic rice
of Cisadane and Rajalele cultivars (cv) was obtained by co-cultivation of rice
callus by 4 strain Agrobacterium tumefaciens containing 4 different binary
vector (pIG 121 Hm, pCAMBIA 1301, pTOK 233 and pMOG22 BarGus). The transgenic
plantlets were acclimatized and grown in the growth chamber to investigate
their agronomic characteristic in comparison to control plants. The results
showed that there was no difference in the number of spikelet and
seeds/spikelet among different transgenic lines co-cultivated by different
strains. The number of seed/spikelet of transgenic rice cv. Rojolele and
Cisadane compared to their control did not show significant difference.
However, the total number of spikelet was different only for cv. Rojolele.
Kata Kunci: Co-cultivation, Agrobacterium tumefaciens, transgenic,
transformation, agronomic characters
Penulis: Pupita Deswina, Inez
H. Slamet Loedin
Kode Jurnal: jpbiologidd110264
