Respon Imun Humoral Pada Larva Helicoverpa armigera hubner (Lepidoptera: Noctuidae) Pasca Infeksi HaNPV (Helicoverpa armigera Nuclear Polyhedrosis Virus) dan Keturunannya
Abstract: NPV is
one of the
biological insecticides that
kill the target
by infecting their
cells including hemocytes. Hemocytes
play a key
role on defense
mechanism against pathogens
like NPV. This
defense mechanism may cause the insect to become more tolerant as well
as to chemical insecticides. The tolerances of
insect to NPV
could be induced
by humoral immune
system (HIS). The
HIS can be
recorded by watch prophenoloxidase (PPO) activity or the
presence of antibacterial compound (AC). Based on that assumption, this
research was conducted to observe the alteration of PPO and AC due to sublethal infection of
HaNPV (Helicoverpa armigera nuclear
polyhedrosis viruses) on
Helicoverpa armigera larvae
and their offspring. PPO was
detected as PO
and measured spectrometrically on
microplate-reader. Protein concentration
was measured based on the color which appears due to protein-CBG (PO and
Bradford reagent) bond at 570 nm. Protein
concentration was calibrated
with BSA. The
occurrence of antibacterial
activity was tested
using modified Hoffman method.
Inhibition zones were
used to measure
the antibacterial activity
from E. coli (Gram
negative) and B.
cereus (Gram positive).
Immune responses were
observed on the
fifth instar Helicoverpa armigera
after 24 hpi by
HaNPV
(NPV-P) and their
offspring (NPV-F1 and
NPV-F2). Also, immune response on
control larvae (K-P) were monitored and their filial (K-F1 and K-F2). The titer
of PO enzyme were not
significantly different for
each generation. There
was no inhibition
zone on antibacterial activity test in every
generation on NPV-P and K-P larvae.
Keywords: Helicoverpa
armigera, HaMNPV, humoral
immune system, prophenoloxidase (PPO), antibacterial activity
Penulis: Nani Kurnia
Kode Jurnal: jpbiologidd110293
