Pengaruh Ekstrak Kopi Robusta (Coffea robusta) sebagai Penghambat Pertumbuhan Staphylococcus aureus

Abstract: Chlorogenic and caffeine acid, which is a non-volatile organic acids in coffee, which can prevent the growth of some gram-positive and gram-negative bacteria including  Escherichia coli,  Salmonella typhi,  Pseudomonas aeruginosa, Pseudomonas  fluorescens,  Staphylococcus  aureus,  Bacillus  cereus,  Lactobacillus  bulgaricus, Streptococcus lactis  and  Streptococcus faecalis.Subject  used in this study was  Staphylococcus aureus  were divided into 5 groups, where each group consisting of 8 repetitions. The first group (R100) Robusta coffee extract at a dose of 100%, the group R50 Robusta coffee extract at a dose of 50%, the group R25 Robusta coffee extract at a dose of 25% and group R12,5 Robusta coffee extract at a dose of 12.5%  and the control group without Robusta.  Data analysis  technique using Kolmogorov-Smirnov normality test, homogeneity test using Levene Test, to determine whether there is a difference using Kruskal Wallis test, and to determine significant differences  using  Mann  Whitney.  The  results  showed  that  the  higher  the  extract  robusta  coffee  (Coffee Robusta), the greater the inhibition of the growth of Staphylococcus aureus, the R100 groups is equal to 7.95 mm. Then successive groups of 5.68 mm R50, R25 group of 4.14 mm, 3.69 mm for R12,5 group, and group C (control) had an average value that is the smallest diameter of inhibition zone measuring 5 mm in diameter paper disc.
Keywords:  robusta coffee, chlorogenic, caffeine acid, Staphylococcus aureus
Penulis: Muhammad Ainul Yaqin, Mumun Nurmilawati
Kode Jurnal: jpbiologidd150329

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