Pemakaian Sel Hela dalam Uji Sitotoksisitas Fraksi Ethanol Biji Mimba (Azadirachta indica)
Abstrak: Traditionally, the
neem (Azadirachta indica) seeds have been used as bioinsecticide. It was also
used and known for long time as a fungicide and anti bacterial agents.
Pre-toxicity experiment using brine shrimp lethality test (BSLT) method
indicated that the ethanol fraction of the neem seeds was toxic to the shrimp
larvae with LC50 38.79 μg/ml. This experiment was done to further investigate
the possible role of ethanol fraction of neem (A. indica) seeds as an anti
cancer agent using HeLa cell lines. Experiment was initiated by extracting the
seeds using n-hexane by maceration. The macerate was then macerated further
using 70% ethanol as solvent. The ethanol fraction was used in cytotoxicity
assay using (cervix cancer) HeLa cell lines. Cytotoxicity assay was done by
using direct counting method. The amount of living cells were observed and
counted, death percentage was determined and probit analysis used to determine
the LC50 value. LC50 value for ethanol fraction from the seeds of neem (A.
indica) was 10.95 μg/ml for 24 hours; 7.33 μg/ml for 48 hours and 4.37 μg/ml
for 72 hours based on cytotoxicity analysis using HeLa cells. This experiment
concluded that the neem (A. indica) seeds have cytotoxicity toward HeLa cell
lines which confirmed the previous toxicity experiment using brine shrimp
lethality test (BSLT) method. Overall, this experiment indicated that neem (A.
indica) seeds have anticancer activity.
Penulis: Ira Djajanegara,
Priyo Wahyudi
Kode Jurnal: jpbiologidd090069
