Media Alternatif untuk Pertumbuhan Bakteri Menggunakan Sumber Karbohidrat yang Berbeda
Abstract: The high cost of
instant media such as nutrient agar encourage researchers to find an
alternative media from raw
materials that are
easily available and
cheap. This study
aims to determine
of using a
source of carbohydrates to used
as an alternative media. The source of carbohydrates that can be used is Canna
edulis, Dioscorea esculenta, and
Maranta arundinaceae and
the results were
compared with nutrient
agar. The sample used are 300 gr
of tubers with addition 10 gr of sugar and 15 gr of agar in 1000 ml of
destilled water.This study used a Completely Randomized Design (CRD) two
factors. The first factor is a type of bacteria: Escherichia coli and Staphylococcus aureus and the second factor is the types of
alternative media: Canna edulis,
Dioscorea esculenta, and Maranta arundinaceae. The bacteria were inoculated
with a spread plate method and incubated at 37 ° C for 24 hours. the parameters
are a number of population and the size of the bacterial colonies.The result
showed that in Escherichia coli, the
highest population is in the Canna
edulis(7.56x107 CFU/ml), Dioscorea esculenta (6,86x107 CFU/ml),
nutrient agar media (6.21x107 CFU/ml), and the last is
Maranta arundinaceae (5.49x107 CFU/ml) while
in the the
Staphylococcus aureus, the
highest population is in the Canna edulis (8.17x107 CFU/ml),
Maranta arundinaceae (5.13x107 CFU/ml), Dioscorea esculenta (4.08x107
CFU/ml), and the last is nutrient agar media (4.02x107 CFU/ml).
Based on the size of the bacteria, the best result is in the Dioscorea esculenta, because the bacterial
colonies are large and looks more clear. The results showed that the media
of Canna edulis, Dioscorea esculenta,
and Maranta arundinaceaecan be used as
an alternatives media for bacterial growth and the most optimal media is Dioscorea esculenta.
Penulis: Anisah, Triastuti
Rahayu
Kode Jurnal: jpbiologidd150331
