Isolasi Senyawa Saponin dari Mangrove Tanjang (Bruguiera gymnorrhiza) dan Pemanfaatannya sebagai Pestisida Nabati pada
ABSTRACT: Bruguiera
gymnorrhiza is a
mangrove plant often
called tanjang containing
several chemical compounds
such as saponins, alkaloids,
tannins, flavonoids and polyphenols. The purpose of this study was to isolate
and determine the toxicity of the methanol extract of bark, leaves and flowers
of B. gymnorrhiza as a plant pesticide.
Samples were extracted by maceration and soxhletation methods using methanol as
solvent. Maceration and soxhletation were done for 24 h and 6 h,
respectively. From distillation of
maceration treatment was obtained 20.28% (bark), 15.95% (leaves) and 19.69%
(interest). In soxhletation there were concentrations of 27.01%, 29.68% and 16,
46%. The results of the foam
test and reagent-Buchard Lieberman
(LB) showed that
only bark and
flowers contain saponins. Toxicity tests on mosquito larvae
with flower extract by maceration was more toxic than bark; on the contrary
bark extract was more toxic than flower extract by soxhletation. SPSS analysis
showed LC50 values for flower extracts was 723.6
ppm and for
bark extract was
673.9 ppm. Both
bark and flower
extracts containing saponins
can be categorized as highly
toxic (<1000 ppm). Therefore it can be used as a botanical pesticide against
mosquito larvae.
Penulis: Larva Nyamuk, ALOWISYA
F. LIEM, ELIZABETH HOLLE, IVONE Y. GEMNAFLE DAN SARAH WAKUM
Kode Jurnal: jpbiologidd130647