Isolasi Senyawa Saponin dari Mangrove Tanjang (Bruguiera gymnorrhiza) dan Pemanfaatannya sebagai Pestisida Nabati pada

ABSTRACT: Bruguiera gymnorrhiza  is  a  mangrove  plant  often  called  tanjang  containing  several  chemical  compounds  such  as saponins, alkaloids, tannins, flavonoids and polyphenols. The purpose of this study was to isolate and determine the toxicity of the methanol extract of bark, leaves and flowers of  B. gymnorrhiza as a plant pesticide. Samples were extracted by maceration and soxhletation methods using methanol as solvent. Maceration and soxhletation were done for 24 h and 6 h, respectively.  From distillation of maceration treatment was obtained 20.28% (bark), 15.95% (leaves) and 19.69% (interest). In soxhletation there were concentrations of 27.01%, 29.68% and 16, 46%. The results of  the  foam  test  and  reagent-Buchard  Lieberman  (LB)  showed  that  only  bark  and  flowers  contain  saponins. Toxicity tests on mosquito larvae with flower extract by maceration was more toxic than bark; on the contrary bark extract was more toxic than flower extract by soxhletation. SPSS analysis showed LC50  values  for flower extracts was  723.6  ppm  and  for  bark  extract  was  673.9  ppm.  Both  bark  and  flower  extracts  containing  saponins  can  be categorized as highly toxic (<1000 ppm). Therefore it can be used as a botanical pesticide against mosquito larvae. 
Key words: mosquito larvae, mangrove tanjang (B. gymnorrhiza), saponins, pesticide
Penulis: Larva Nyamuk, ALOWISYA F. LIEM, ELIZABETH HOLLE, IVONE Y. GEMNAFLE DAN SARAH WAKUM
Kode Jurnal: jpbiologidd130647

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