Isolasi dan Identifikasi Kapang Kontaminan pada Permen Labu Kuning dari Sumbawa Besar
Abstract: Pumpkin
candy is a
sort of pumpkin
fruit processed products
that is typical
food from Sumbawa
Besar. Pumpkin candy contains carbohydrates, fats, protein, fiber,
and minerals. The mold can contaminated and degradated the pumpkin candy
compounds, thus lowering the quality of the pumpkins candy. The purpose of this
research were: 1) to identify the species of
contaminant mold on candy pumpkin; 2) to determining the most dominant
species on the pumpkins candy. The research were conducted at the Microbiology
Laboratory, Departement of Biology, State University of Malang. The pumpkin
candy sample was from Sumbawa Besar. The samples stored in a sterile jars for 8
days. The 25 grams of pumpkin candy were granded, then dissolved in 225 ml
of 0,1% peptone to obtain a suspension
with a dilution rate 10-1. Then the suspension is diluted again gradually
in order to
obtain a suspension
with a dilution
rate 10-2, 10-3,
10-4, 10-5, and 10-6. The suspension on each level of dilution was
inoculated on a medium plate Czapek Agar (CA), and then incubated at 25-27oC
for 7x24 hours. Identification
contaminant molds was done by observing the colony morfology and
microscopic characteristic then referred to the book of mold identification.
The results showed: 1) there are 11 species
of contaminants mold
that grow in
pumpkin candy. The
contaminant mold species
are Aspergillus terreus Thorn, Penicillium brevicompactum Dierckx,
Penicillium paraherquei Abe ex G. Smith, Aspergillus niger Van Tieghem,
Penicillium frequetans Westling, Aspergillus candidus Link, Cladosporium herbarum (Pers.)
Link ex Gray,
Cladosporium cladosporioides (Fres.)
de Vries, Nigrospora
oryzae, Penicillium verrucosum
Dierckx var corymbeferum (Westling) Samson et al., and Penicillium camemberti Thorn; 2) the dominant
contaminant mold species in pumpkin candy samples is Cladosporium.
Penulis: Utami Sri Hastuti,
Linda Hapsari, Henny Nurul Khasanah
Kode Jurnal: jpbiologidd150333
